Optimizing bacterial strain design for increased protein production

 

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unfolded protein in inclusion body   deletions and overexpressions

Fig.1                                                      Fig.2

 

Keywords

  • Genetic design
  • Bacterial chaperones
  • « Hard to produce » protein

 

Technology market

Protein Production Industry

Until now, no or very little efforts have been made regarding the genotype of the bacterial strain used for recombinant protein production concerning:

  • Deletion of proteases
  • Overexpression of chaperones
  • Modification of the exportation system
  • Vectors used for the overexpression
  • Removal of contamiants proteins
  • ...

 

The Collet Lab Expertise

  • Physiological comprehension of molecular processes and modifications of the bacterial genetic background
  • Production in the periplasm: Disulfide bridges formation, Glycoproteins, membrane proteins, …
  • Chaperones and repairs systems

 

The UCL collaboration offer

On demand optimization of already established strains (BL21,…):

  • by adding specific genetic features (chaperones, proteases, S-S formation proteins, ...) (Fig. 1)
  • through overexpression or deletion of dedicated genes to enhance recombinant protein production in the periplasm or the cytoplasm of bacteria (Fig. 2)

The lab offers a large panel of to increase the production of hard to produce proteins

 

Preferred partnership

Fee for service to compagnies in need to increase the protein yield  in E. coli

 

Interested to benefit from these services?
Please contact :

Marlène DUBUISSON
Technology Transfer Advisor 
+32 10 47 25 42


www.ltto.com